维亚根/蛋白酶K粉200mg/506-PKP
商品编号:
506-PKP
品牌:
Viagen Biotech
市场价:
¥3100.00
美元价:
1860.00
产品分类:
其它缓冲液
公司分类:
Other_buffers
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Details
Proteinase K is an endoproteolytic enzyme that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a nonspecific serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.
Applications: Proteinase K effectively digests native proteins. It can be used to inactivate DNases and RNases when isolating DNA and RNA from tissues or cells.
Usage Conditions:
• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At high temperatures—optimum digestion temperature is 65°C
Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.
Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases
品牌介绍
Viagen生產快速溶解細胞組織樣品,提供DirectPCR Lysis Reagents多種樣品,包含老鼠尾巴、耳朵、細胞,卵黃囊genomic DNA,適合各廠牌的PCR酵素快速分析。只需要1 µl lysates.即可快速執行genotyping分析。
原廠連結 http://www.viagenbiotech.com/
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