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当前位置: 首页 > 产品中心 > Other_buffers > 维亚根/蛋白酶K溶液25ml(20mg/ml)/503-PK
商品详细维亚根/蛋白酶K溶液25ml(20mg/ml)/503-PK
维亚根/蛋白酶K溶液25ml(20mg/ml)/503-PK
维亚根/蛋白酶K溶液25ml(20mg/ml)/503-PK
商品编号: 503-PK
品牌: Viagen Biotech
市场价: ¥8900.00
美元价: 5340.00
产地: 美国(厂家直采)
公司:
产品分类: 其它缓冲液
公司分类: Other_buffers
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Details

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.

DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed, and therefore when it is difficult to weigh Proteinase K powder. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.

Other applications: Proteinase K digests native proteins very effectively. It can therefore be used to remove DNases and RNases when isolating DNA and RNA from tissues or cells.

Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C

Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.

Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases

品牌介绍
Viagen生產快速溶解細胞組織樣品,提供DirectPCR Lysis Reagents多種樣品,包含老鼠尾巴、耳朵、細胞,卵黃囊genomic DNA,適合各廠牌的PCR酵素快速分析。只需要1 µl lysates.即可快速執行genotyping分析。 原廠連結  http://www.viagenbiotech.com/